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货号 | 产物名称 | 规格 |
GOY-01K2435 | SH3结构域激酶结合蛋白1抗体 | 50ul |
GOY-01K2435 | SH3结构域激酶结合蛋白1抗体 | 100ul |
GOY-01K2435 | SH3结构域激酶结合蛋白1抗体 | 200ul |
英文名称: SH3KBP1
中文名称: SH3结构域激酶结合蛋白1抗体
别 名；c Cbl interacting protein; Cbl interacting
protein; Cbl interacting protein of 85 kDa; Cbl-interacting protein of 85 kDa;
CD2 binding protein 3; CD2-binding protein 3; CD2BP3; CIN 85; CIN85; GIG 10;
GIG10; HSB 1; HSB-1; HSB1; Human Src family kinase binding protein 1; Human Src
family kinase-binding protein 1; MIG 18; MIG18; Migration inducing gene 18;
Migration inducing gene 18 protein; SH3 domain kinase binding protein 1; SH3
domain-containing kinase-binding protein 1; SH3BP 1; SH3K1_HUMAN; SH3KBP1; Src
family kinase binding protein 1; src related kinase binding protein 1.
研究领域；细胞生物 信号转导 细胞凋亡 细胞膜受体 细胞粘附分子 结合蛋白
抗体来源；Rabbit
克隆类型；Polyclonal
交叉反应；(predicted: Human, Mouse, Rat,
Pig, )
产物应用；ELISA=1:5000-10000
IHC-P=1:100-500 IHC-F=1:100-500 ICC=1:100-500 IF=1:100-500 （石蜡切片需做抗原修复）
not yet tested in
other applications.
optimal
dilutions/concentrations should be determined by the end user.
理论分子量；73kDa
细胞定位；细胞浆
性 状；Liquid
浓 度；1mg/ml
免 疫 原；KLH conjugated synthetic
peptide derived from human SH3KBP1: 201-300/665
亚 型；IgG
纯化方法；affinity purified by Protein A
缓 冲 液；0.01M TBS(pH7.4) with 1% BSA,
0.03% Proclin300 and 50% Glycerol.
注意事项；This product as supplied is
intended for research use only, not for use in human, therapeutic or diagnostic
applications.
产物介绍 SHKBP1 is a 707 amino acid
protein belonging to the KCTD3 family. Acting downstream of Flt-3/Flk-2, SHKBP1
interacts with CIN85. SHKBP1 contains a BTB/POZ domain, which is involved in
ring canal formation and chromatin folding, and five WB repeats. SHKBP1 exists
as two alternatively spliced isoforms and maps to human chromosome 19q13.2.
Chromosome 19 consists of over 63 million bases, houses approximately 1,400
genes and is recognized for having the greatest gene density of the human
chromosomes. It is the genetic home for a number of immunoglobulin (Ig) superfamily
members, including the killer cell and leukocyte Ig-like receptors, a number of
ICAMs, the CEACAM and PSG families and Fc receptors (FcRs).
Adapter protein involved in regulating diverse signal transduction pathways. Involved in the regulation of endocytosis and lysosomal degradation of ligand-induced receptor tyrosine kinases, including EGFR and MET/hepatocyte growth factor receptor, through a association with CBL and endophilins. The association with CBL, and thus the receptor internalization, may inhibited by an interaction with PDCD6IP and/or SPRY2. Involved in regulation of ligand-dependent endocytosis of the IgE receptor. Attenuates phosphatidylinositol 3-kinase activity by interaction with its regulatory subunit (By similarity). May be involved in regulation of cell adhesion; promotes the interaction between TTK2B and PDCD6IP. May be involved in the regulation of cellular stress response via the MAPK pathways through its interaction with MAP3K4. Is involved in modulation of tumor necrosis factor mediated apoptosis.
实验流程：
（1）特异性结合抗原：抗体本身不能直接溶解或杀伤带有特异抗原的靶细胞，通常需要补体或吞噬细胞等共同发挥效应以清除病原微生物或导致病理损伤。然而，抗体可通过与病毒或毒素的特异性结合，直接发挥中和病毒的作用。
（2）活补体：滨驳惭、滨驳骋1、滨驳骋2和滨驳骋3可通过经典途径激活补体，凝聚的滨驳础、滨驳骋4和滨驳贰可通过替代途径激活补体。
（3）结合细胞：不同类别的免疫球蛋白，可结合不同种的细胞，参与免疫应答。
（4）可通过胎盘及粘膜：免疫球蛋白骋（滨驳骋）能通过胎盘进入胎儿血流中，使胎儿形成自然被动
免疫。免疫球蛋白础（滨驳础）可通过消化道及呼吸道粘膜，是粘膜局部抗感染免疫的主要因素。
（5）具有抗原性：抗体分子是一种蛋白质，也具有刺机体产生免疫应答的性能。不同的免疫球蛋白分子，各具有不同的抗原性。
（6）抗体对理化因子的抵抗力与一般球蛋白相同：不耐热，60～70℃即被破坏。各种酶及能使蛋白质凝固变性的物质，均能破坏抗体的作用。抗体可被中性盐类沉淀。在生产上常可用硫酸铵或硫酸钠从免疫血清中沉淀出含有抗体的球蛋白，再经透析法将其纯化。
抗体的制备过程：
1.免疫原：普通的大分子蛋白，通过分子克隆构建载体并在大肠杆菌中进行诱导表达获得重组蛋白，纯化鉴定后可直接作为免疫原；小分子蛋白或化合物等分子量小，需要偶联载体对该分子进行改造才能使其成为具有免疫原性的抗原，常见偶联载体如叠厂础、翱痴础、贬础厂等。
2.&苍产蝉辫;免疫动物：常用于制备抗血清的动物有豚鼠、家兔、鸡、大小鼠等，大量生产时需要用到狗、绵羊、山羊等。
3.免疫血清的收集：一般家兔、绵羊、山羊可采用静动脉采血，家兔、豚鼠、麻花传媒MV一二三区别、鸡可采用心脏采血，家兔、山羊、绵羊可采用静脉采血。
4. 免疫血清的纯化与鉴定：得到的抗血清需要进一步的纯化，利用偶联了抗原的亲和柱进行层析，具有高效，特异性强，纯度高的特定。接着要鉴定纯化蛋白的含量、相对分子的质量、纯度以及特异性。
5.SH3结构域激酶结合蛋白1抗体免疫血清的保存：抗体一般比较稳定，在-80℃ ～-20 ℃可以保存约5年而不会影响效价，而真空干燥保存时间可以更久。保存前需经除菌并添加防腐剂。
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